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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Allergy Clin Immunol. 2010 Dec 24;127(6):1400–1407.e4. doi: 10.1016/j.jaci.2010.11.008

Figure 4. Patient origin and chromosomal integrity of the PID-specific iPSCs.

Figure 4

A) PCR amplification followed by DNA sequencing of genomic DNA derived from the PID-specific iPSCs and their parental fibroblasts was performed using specific primers corresponding to the disease-causing mutations for each of the lines, and demonstrated that the PID-specific iPSC lines carry the same disease-causing mutations as their parental fibroblasts. In the case of the first allele (c.256-257del) of the RAG1-mutated patient with Omenn syndrome, genetic identity between patient-derived iPSCs and fibroblasts was demonstrated upon cloning and sequencing of the specific product.

B) PID-specific iPSCs were analyzed for chromosomal integrity by G-banding karyotyping.