Fig. 2.

Design of the conserved primers based on nucleotide sequence conservation of the LMW-GS genes in Xiaoyan 54. Based on the conserved sequences of LMW-GS genes, three sets of conserved primers were designed: LMWGS1, LMWGS2 and LMWGS3. LMWGS1 comprised LMWGS1,2F and LMWGS1R. LMWGS2 contained the pair of primers LMWGS1,2F and LMWGS2R, whereas LMWGS3 comprised the three pairs of conserved primers LMWGS3a (LMWGS3aF and LMWGS3aR), LMWGS3b (LMWGS3bF and LMWGS3bR) and LMWGS3c (LMWGS3cF and LMWGS3cR). All of the conserved primers were matched to the sequences flanking the repetitive sequences. Specifically, all the forward primers were located in the sequences encoding the signal peptide or the N-terminal domain, while the reverse primers were located in the sequences encoding the C-terminal I region