Fig. 3.

Analysis of polymerase chain reaction (PCR) products amplified from Xiaoyan 54 with the conserved primers. a Electrophoresis of the PCR products amplified from Xiaoyan 54 with the conserved primers in an agarose gel. M DNA ladder Marker III (200, 500, 800, 1,200, 2,000, 3,000 and 4,500 bp; Tiangen Biotech Co., Ltd.). b Electropherograms showing capillary electrophoresis separation of the DNA fragments amplified from Xiaoyan 54 with the conserved primers. The horizontal axis shows the size of the detected DNA fragments, while the vertical axis displays the intensity of the signal (i.e. the concentration of DNA fragments in the PCR products). The orange peaks match the standard DNA fragments in the GeneScan 1200 LIZ size standard, while the blue ones representing the DNA fragments in the PCR products, should match theoretically with the LMW-GS genes in Xiaoyan 54. The numbers on the horizontal axis represent the size of the corresponding peak in the GeneScan 1200 LIZ size standard (orange). In Xiaoyan 54, 14 blue peaks (DNA fragments) could be detected with the conserved primer LMWGS1, 16 with LMWGS2 and 16 with LMWGS3 (7 with LMWGS3a, 6 with LMWGS3b and 3 with LMWGS3c). The data shown are representative of four independent sets of PCR amplifications and DNA fragment analyses