Table 1.
Three sets of conserved primers designed based on the 13 LMW-GS gene sequences from Xiaoyan 54 BAC clones and those available in GenBank
| Name | Sequences (5′–3′) |
|---|---|
| LMWGS1 | |
| LMWGS1,2F | |
| LMWGS1,2Fa | ATGAAGACCTTCCTCRTCTTTG |
| LMWGS1R | |
| LMWGS1R-1 | CATGGGTTTAGCTGCTGCA |
| LMWGS1R-2 | CACGGGTTTAGCTGCTGCA |
| LMWGS1R-3 | CATGGGTTTAGTTGCTGCA |
| LMWGS1R-4 | CATGGGTTCAGCTGCTGCA |
| LMWGS2 | |
| LMWGS1,2F | |
| LMWGS1,2Fa | ATGAAGACCTTCCTCRTCTTTG |
| LMWGS2R | |
| LMWGS2R-1 | CAACATTGTTGYTGCATCACAT |
| LMWGS2R-2 | CAACATTGTCGCTGCATCACAT |
| LMWGS2R-3 | CAACATTGTTGCCGCATCACAT |
| LMWGS3a | |
| LMWGS3aF | |
| LMWGS3aF-1 | CCTGGTTTGGAGAAACCATG |
| LMWGS3aF-2 | CCTGGTTTGGAGAGACCATG |
| LMWGS3aR | |
| LMWGS3aR-1a | CATYTGYGACCTAGCAAGACG |
| LMWGS3aR-2a | CATCTGCGACCTAGCAAGAT |
| LMWGS3b | |
| LMWGS3bF | |
| LMWGS3bF-1 | CCTAGCTTGGAGAAACCATT |
| LMWGS3bF-2 | CCTGGCTTGGAGAAACCATC |
| LMWGS3bF-3 | CCTGGTTTGGAGAGACCATC |
| LMWGS3bF-4 | CCTGGTTTGGAGAAACCATC |
| LMWGS3bR | |
| LMWGS3bRa | CATTTGYGACCTAGCAAGACT |
| LMWGS3c | |
| LMWGS3cF | |
| LMWGS3cF | GCCGTTGCGCAAATTTCAC |
| LMWGS3cR | |
| LMWGS3cRa | CATTTGTGACCTAGCAAGACATC |
LMWGS1 and LMWGS2 each contains only one pair of primers, and both share the same forward primer, LMWGS1,2F, whereas LMWGS3 comprises three pairs of primers (LMWGS3aF and LMWGS3aR, LMWGS3bF and LMWGS3bR and LMWGS3cF and LMWGS3cR). The primers with unique nucleotides (underlined) were all synthesised and then mixed to work as the forward or reverse primers
a5′ terminal of the primer was labelled with 6FAM