Table 2.

Size of the DNA fragments amplified from Xiaoyan 54 with three sets of conserved primers and subsequently detected with the Applied Biosystems 3730 DNA Analyzer

Gene	LMWGS1		LMWGS2		LMWGS3a		LMWGS3b		LMWGS3c		Accession no.c	Identityd (%)
	Theoa	Expb	Theo	Exp	Theo	Exp	Theo	Exp	Theo	Exp
LGS2-477 e,f	377	370	485	477	366	368	–	–	–	–	AJ519836	98
D3-4	392	385	500	492	381	383	–	–	–	–	FJ755311	100
D3-5 f	399	393	507	499	388	390	–	–	–	–	FJ755312	100
D3-7	401	394	509	501	390	393	–	–	–	–	FJ755314	100
A3-1	410	402	518	509	399	402	–	–	–	–	FJ755302	100
D3-2	440	432	548	538	429	432	–	–	–	–	FJ755315	100
LGS2-590 e,f	493	Ng	601	590	–	–	–	–	512	N	FJ549941	99
D3-6	536	525	644	632	525	531	–	–	–	–	FJ755313	100
B3-1	542	530	650	637	–	–	531	537	–	–	FJ755306	100
LGS2-655 e,f	561	549	669	655	–	–	550	556	–	–	EU369731^6	100
A3-3 f	578	566	686	672	–	–	–	–	597	605	FJ755305	100
A3-2	581	568	689	675	–	–	–	–	600	608	FJ755304	100
D3-1	587	575	695	682	–	–	576	583	–	–	FJ755310	100
D3-3	590	N	698	N	–	–	579	586	–	–	FJ755316	100
LGS2-690 e,f	596	584	704	690	–	–	585	593	–	–	AY585349	97
A3-4	680	664	788	768	–	–	–	–	699	715	FJ755303	100
B3-2	704	688	812	792	–	–	693	707	–	–	FJ755309	100
B3-3 f	–h	–	–	–	–	–	–	–	–	–	FJ755307	–

^aTheoretical size of DNA fragments

^bExperimental size of DNA fragments

^cFJ755302–FJ755307 and FJ755309–FJ755316 were identified in Xiaoyan 54 BAC clones reported by Dong et al. (2010)

^dNucleotide sequence identity of the DNA fragments to previously reported LMW-GS genes

^eThe new genes from Xiaoyan 54 identified in the present study

^fThe genes were not expressed in the developing seeds of Xiaoyan 54

^gNot detected

^hNot applicable