Table 1.
Selection round | Input phagea (pfu) | Recovered phageb (pfu) | Binding time (min) | Number of washes |
---|---|---|---|---|
1 | 4 × 1010 | 4.3 × 107 | 90 | 2 |
2 | 2 × 1011 | 9.1 × 107 | 90 | 5 |
3 | 2 × 1011 | 4.3 × 107 | 30 | 10 |
4 | 2 × 1011 | 4.4 × 106 | 15 | 10 |
5 | 2 × 1011 | 3.7 × 107 | 15 | 10 |
a, bPhage counts are given in plaque-forming units (pfu). For the first round, 0.1% TBST was used as the washing buffer. For the second through fifth round, 0.5% TBST was used