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. 2011 Jan 23;33(5):1069–1073. doi: 10.1007/s10529-011-0519-6

Table 1.

Summary of phage selection conditions

Selection round Input phagea (pfu) Recovered phageb (pfu) Binding time (min) Number of washes
1 4 × 1010 4.3 × 107 90 2
2 2 × 1011 9.1 × 107 90 5
3 2 × 1011 4.3 × 107 30 10
4 2 × 1011 4.4 × 106 15 10
5 2 × 1011 3.7 × 107 15 10

a, bPhage counts are given in plaque-forming units (pfu). For the first round, 0.1% TBST was used as the washing buffer. For the second through fifth round, 0.5% TBST was used