Fig. 4.

Effect of resveratrol on AGEs-induced oxidative stress in RIN-5F cells. Intracellular ROS in RIN-5F cells were measured with DCFH-DA fluorescent probe and a flow cytometer. A: Effect of resveratrol on basal ROS in RIN-5F cells. B: Effects of AGE1 and AGE2 on intracellular ROS levels in RIN-5F cells after 3 h treatment with AGEs. BSA alone (CNT) was used as control. C, D: Effect of resveratrol on AGEs-induced oxidative stress. E: Graph of statistically processed data. Each value represents the mean ± SEM for 4 assays. Values not sharing a common letter are significantly different at p < 0.001 by Tukey-Kramer multiple comparisons test.