Table 1.
Time constants of recovery of rat neurones under the different experimental condition
| Condition | τ (s) | Rate (s−1) | Isol. rates (s−1) | Calculation of rates for the different pathways | |||
|---|---|---|---|---|---|---|---|
| ACSF | 7.85 ± 2.41 (84) | 0.1273 | |||||
| CDC-ACSF | 6.87 ± 2.36 (44) | 0.1456 | 0.1456 | ||||
| CDC-ACSF | 7.01 ± 1.67 (12) | 0.1426 | |||||
| With bumet. | 8.60 ± 1.61 (12) | 0.1163 | 0.0263 | 0.0263 | −0.0263 | ||
| CDC-ACSF | 7.12 ± 2.97 (8) | 0.1405 | |||||
| With furosemide | 16.57 ± 6.69 (8) | 0.0603 | 0.0801 | ||||
| CDC-ACSF | 6.58 ± 2.72 (7) | 0.1521 | |||||
| With 9AC | 8.44 ± 4.16 (7) | 0.1185 | 0.0336 | 0.0336 | −0.0336 | ||
| CDC-5 mm K+ | 7.13 ± 2.95 (5) | 0.1402 | |||||
| CDC-2.5 mm Cs+ | 12.23 ± 4.45 (5) | 0.0818 | 0.0584 | ||||
| CDC/nitrate | 21.06 ± 6.06 (17) | 0.0475 | 0.0475 | 0.0475 | |||
| With furosemide | 28.60 ± 4.56 (3) | 0.0350 | |||||
| CDC/nitrate | 19.32 ± 5.22 (4) | 0.0518 | |||||
| With 9AC | 41.68 ± 8.49 (4) | 0.0240 | 0.0278 | −0.0278 | −0.0197 | ||
| rate: | 0.0263 | 0.0336 | 0.0197 | 0.0659 | |||
| pathway: | NKCC1 | ClC2 | AE | KCC2 | |||
The time constants in control CDC-ACSF differ in some of the subgroups from the ensemble average. The rates were therefore calculated for each experimental group as difference in rate with and without the drugs. On the right hand the calculation of the rates of the individual pathways is presented. From the different rates obtained with 9AC for Cl− and NO3− extrusions, a relative permeability of ClC2 of Cl−vs. NO3− was calculated (0.83). Note that three neurones were not included in the average of the CDC-ACSF group, because the values were considered outliers (above the mean +2 s.d.).