Table 2.
Time constants of recovery of TLE neurones under the different experimental condition
| Condition | τ (s) | Rate (s−1) | Isol. rates (s−1) | Calculation of rates for the different pathways | |||
|---|---|---|---|---|---|---|---|
| ACSF | 20.32 ± 11.38 (85) | 0.0492 | |||||
| CDC-ACSF | 19.02 ± 9.63 (62) | 0.0526 | 0.0526 | ||||
| CDC-ACSF | 19.36 ± 4.48 (8) | 0.0517 | |||||
| With bumet. | 24.05 ± 3.15 (8) | 0.0416 | 0.0101 | 0.0101 | −0.0101 | ||
| CDC-ACSF | 15.45 ± 6.02 (8) | 0.0647 | |||||
| With furos. | 44.10 ± 11.43 (8) | 0.0227 | 0.0420 | ||||
| CDC-ACSF | 14.80 ± 4.47 (8) | 0.0676 | |||||
| With 9AC | 16.45 ± 4.95 (8) | 0.0608 | 0.0068 | 0.0068 | −0.0056* | −0.0068 | |
| CDC-ACSF-5 mm K+ | 15.04 ± 4.69 (9) | 0.0665 | |||||
| CDC-ACSF-2.5 mm Cs+ | 17.48 ± 5.97 (9) | 0.0572 | 0.0093 | ||||
| CDC/nitrate | 35.48 ± 17.29 (7) | 0.0282 | 0.0282 | 0.0282 | −0.0226 | ||
| rate | 0.0101 | 0.0068 | 0.0226 | 0.0132 | |||
| pathway | NKCC1 | ClC2 | AE | KCC2 | |||
The time constants in control CDC-ACSF differ for some of the subgroups from the ensemble average. The rates were therefore calculated for each experimental group as difference in rate with and without the drugs. On the right hand the calculations of the rates of individual pathways are presented. To obtain the estimate for the AE, the NO3− rate of ClC2 has to be subtracted (see Table 1). To compensate for the lack of NO3− transport rate via ClC2, a calculated NO3− extrusion rate (indicated by the asterisk) was used (ClC2 rate for Cl− multiplied by 0.83, determined experimentally in rat neurones).