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. 2011 Apr 4;193(1):61–70. doi: 10.1083/jcb.201007003

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© 2011 Gu et al.

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Figure 3. — After macropinosome disassembly, internalized integrin β3 colocalizes with early endosomes (EEA1, Rab5, and Rab4) and recycling endosomes (Rab11). (A−D) Primary mouse fibroblasts were stimulated with or without PDGF-BB, fixed, and IF stained. (E) Series of overlay images in A−D were analyzed by a colocalization assay module in MetaMorph software. Percentages of integrin β3 that colocalized with EEA1, Rab5, Rab4, and Rab11 are plotted (n = 10). Error bars represent means ± SD. ***, P < 0.05. Bars: (overlay) 20 µm; (zoom) 5 µm.