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. 2011 Apr 4;193(1):97–108. doi: 10.1083/jcb.201011083

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© 2011 Harrigan et al.

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Figure 2. — 53BP1 colocalizes with components of OPT domains. (A) Immunofluorescence was performed in BJ primary or BJ hTERT (PTFγ) fibroblasts with mouse anti-53BP1 and rabbit anti–Oct-1, anti-PTFδ, or anti-PTFγ, and rabbit anti-53BP1 and mouse anti-PML antibodies as indicated. Analysis of >100 cells revealed that 88% of 53BP1 nuclear bodies contained at least one PML body. (B) Experiments were performed as in A with U2OS cells and the indicated antibodies. (C) Immunofluorescence was performed in BJ primary fibroblasts with mouse anti-53BP1 and rabbit anti-DDX1 antibodies. (D) BJ hTERT fibroblasts were exposed to 1.5 Gy of ionizing radiation and fixed 1 h later. Immunofluorescence was performed with rabbit anti-PTFδ and mouse anti-53BP1 antibodies. Bars, 10 µm.