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. 2011 Apr 4;193(1):97–108. doi: 10.1083/jcb.201011083

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© 2011 Harrigan et al.

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Figure 4. — 53BP1-OPT domains do not associate with regions of active transcription and are dependent on DNA. (A) Immunofluorescence was performed in BJ primary fibroblasts with mouse anti-53BP1 and rabbit anti-Pol II pS2 (top) or rabbit anti-53BP1 and mouse anti-Pol II pS5 (bottom) antibodies. (B) BJ primary fibroblasts were incubated for 60 min with FlU and immunofluorescence was performed with rabbit anti-53BP1 and mouse anti-BrdU antibodies. (C) BJ primary fibroblasts were incubated in the absence or presence of DNase I as indicated for 10 min. Immunofluorescence was performed with rabbit anti-53BP1 or mouse anti–Lamin A/C antibodies. DAPI and Lamin A/C were positive and negative controls, respectively. Bars, 10 µm.