Figure 1.

Ctf4 is conserved across species and interacts with DNA replication proteins. A. Alignment of Ctf4 from different organisms shows a conserved WD40 domain and central domain, as well as an HMG domain conserved only in vertebrates. B. Serial dilutions of PJ69α yeast two-hybrid reporter strain with the indicated fusion constructs. Drosophila Ctf4 fused to GAD interacts with Drosophila GBK fusions of Psf1, Psf2, Mcm2, and Pol alpha respectively as evidenced by growth on media lacking histidine, while no growth occurs in the control.