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. 2011 Apr 6;12:13. doi: 10.1186/1471-2199-12-13

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Copyright ©2011 Gosnell and Christensen; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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GAL4/UAS-driven RNAi system in Drosophila for knockdown of CTF4. A. Crossing scheme for the generation of CTF4 knockdown flies and rtPCR of resulting knockdown. GFP positive larvae are sibling controls for the non-glowing CTF4 knockdown larvae. Transcript levels are reduced by 87% in RNAi flies compared to sibling controls. Analysis was carried out on these groups as indicated. B. Alternate strategy for CTF4 knockdown using a heat-shock promoter upstream of the RNAi sequence. Analysis by rtPCR shows an 81% reduction in CTF4 transcript compared to wild-type controls.