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Ctf4 is required for viability. A. Graph showing survival at the third-instar, pupation, and eclosion for CTF4 knockdown and sibling control showing that CTF4 is required for successful eclosion. Maternal loading is no longer sufficient to augment knockdown of Ctf4 at pupation. B. SEM images of aberrant phenotype in Ctf4 knockdown eclosion survivors.
