Figure 5.

APC8 Is Required for miR159 Biogenesis during Male Gametophyte Development.

(A) miR159 accumulation in Col-0, apc8-1, and APC8 apc8-1 (the pAPC8:APC8-YFP construct was introduced into apc8-1). Total RNAs were extracted from inflorescences. U6 was the loading control.

(B) Expression of MIR159a (light gray), MIR159b (dark gray), and MIR159c (black) in pollen from Col-0, apc8-1, apc13-2, and HTR10:APC8 (line 8-2). UBIQUITIN5 (UBQ5) was the loading control. All measurements represent the average of three biological replicates with error bars representing the se.

(C) Expression patterns of ProMIR159a, b, c-GFP during pollen development in wild-type and apc8-1 plants. Fifteen individual T1 plants in the wild type or the apc8-1 background were examined. Panels (top to bottom) show three progressive stages: UP, uninucleate microspores; BP, bicellular pollen; TP, tricellular pollen. A representative DAPI-stained image for each stage is shown. Bar = 10 μm.

[See online article for color version of this figure.]