Figure 8.

CYCB1;1 Expression Is Increased in Male Gametophytes of apc/c Mutants.

(A) Expression of CYCB1;1 in mature pollen from Col-0, apc8-1, and apc13-2. UBIQUITIN5 (UBQ5) was the loading control. All measurements represent the average of three biological replicates with error bars representing the se.

(B) Subcellular localization of CYCB1-GFP during pollen development in wild-type and apc8-1 plants harboring pCYCB1;1:CYCB1-GFP. Fifteen individual T1 plants in the wild type or the apc8-1 background were examined. DAPI staining of uninucleate microspores (UP; [a] and [c]), bicellular pollen (BP; [e] and [g]), and tricellular pollen (TP; [i] and [k]). GFP was examined in uninucleate microspores ([b] and [d]), bicellular pollen ([f] and [h]), and tricellular pollen ([j] and [l]). CYCB1;1-GFP in apc8-1 was detected weakly in generative cell nuclei (arrow) of bicellular pollen (i) and obviously accumulated in both the vegetative nucleus (arrowhead) and sperm cell nuclei (arrow) of tricellular pollen (l). Bar = 10 μm.

[See online article for color version of this figure.]