Table 3.
Lignin Content and Enzymatic Hydrolysis of Mature Stems from Laccase Mutants
| Lignin Content |
Enzymatic Hydrolysis |
||||
| Growth Conditions | Line | KL% | ASL% | Weight Loss% | Released Glc% |
| Long-day (no phenotype) | Wild type | 20.05 ± 0.18 (100) | 1.37 ± 0.07 | 28.2 ± 1.0 (100) | 9.0 ± 0.3 |
| lac4-2 | 17.35 ± 0.13 (87) | 1.70 ± 0.01 | 31.4 ± 0.1 (111) | 9.3 ± 0.9 | |
| lac17 | 17.26 ± 0.12 (86) | 1.63 ± 0.05 | 28.2 ± 0.5 (100) | 9.7 ± 0.4 | |
| lac4-1 lac17 | 15.37 ± 0.02 (77) | 1.95 ± 0.02 | 36.9 ± 0.1 (131) | 11.0 ± 0.0 | |
| lac4-2 lac17 | 12.66 ± 0.16 (63) | 1.70 ± 0.01 | 52.6 ± 0.4 (187) | 23.4 ± 0.4 | |
| Continuous light (semidwarf phenotype for lac4-2 lac17) | Wild type | 20.13 ± 0.09 (100) | 1.04 ± 0.02 | 29.4 ± 0.4 (100) | 9.9 ± 0.5 |
| lac4-1 | 18.42 ± 0.01 (92) | 1.10 ± 0.03 | 35.8 ± 0.6 (122) | 13.6 ± 0.5 | |
| lac4-2 | 18.27 ± 0.10 (91) | 0.96 ± 0.01 | 35.9 ± 1.1 (122) | 13.1 ± 0.7 | |
| lac17 | 17.97 ± 0.13 (89) | 1.20 ± 0.01 | 29.0 ± 0.4 (99) | 9.4 ± 0.3 | |
| lac4-1 lac17 | 16.22 ± 0.00 (81) | 1.10 ± 0.01 | 30.6 ± 0.5 (104) | 11.0 ± 0.2 | |
| lac4-2 lac17 | 12.24 ± 0.01 (61) | 1.56 ± 0.01 | 61.9 ± 0.5 (210) | 30.5 ± 0.6 | |
The Klason lignin (KL) and the acid-soluble lignin (ASL) contents are expressed as a percentage of the extract-free samples, in terms of weight. Data are mean values and standard errors from duplicate experiments. The values in parentheses are the percentages relative to the corresponding control.