Figure 5.

Induction of Target Gene Expression by a Phosphorylation-Mimicking Mutant of WRKY8.

(A) Expression levels of NADP-ME (top) and HMGR2 (bottom) induced by MEK2^DD (left) or P. infestans (right) were compromised by TRV:WRKY8 or TRV:SIPK/WIPK. Data are means ± sd from three independent experiments.

(B) Expression of NADP-ME and HMGR2 in response to WRKY8 variants. Total RNAs were extracted from N. benthamiana leaves 48 h after agroinfiltration and were used for qRT-PCR. Data are means ± sd from at least three independent experiments. Anti-HA antibody was used to detect accumulation of WRKY8-HA variants. Protein loads were monitored by CBB staining of the bands corresponding to RBCL. WT, wild type.

*P < 0.05 and **P < 0.01; two-tailed Student’s t test.

[See online article for color version of this figure.]