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. 2011 Mar 15;23(3):984–999. doi: 10.1105/tpc.111.083071

Figure 7.

Figure 7.

ech2 ibr10 Displays Decreased Auxin Reporter Activity in Response to Active Auxins.

(A) Seven-day-old light-grown wild-type (Wt) and ech2-1 ibr10-1 seedlings carrying DR5-GUS were transferred to medium supplemented with ethanol (mock) or the indicated auxin and were incubated for 2 h at 22°C prior to staining for GUS activity for 1.5 h. Bar = 100 μm.

(B) Mean GUS activity (±se; n = 16) of 7-d-old light-grown wild-type and ech2-1 ibr10-1 seedlings carrying DR5-GUS treated for 2 h with ethanol (mock treatment; 0 μM auxin) or the indicated auxin.

(C) Mean root lengths (±se; n = 13) of 8-d-old light-grown wild-type and ech2-1 ibr1-2 ibr3-1 ibr10-1 seedlings grown on medium supplemented with ethanol (mock treatment; 0 nM IAA) or various concentrations of IAA.