Table 3.
Mean measured values (ng/mL) and relative standard deviations (RSD) by triplicate analysis of spiked bovine serum samples
| 3,5-T2 | 3,3′-T2 | T3a | rT3 | T4a | |
|---|---|---|---|---|---|
| 100 ng/mL | |||||
| Intraday Mean | 106.3 | 105.5 | 115.8 | 106.9 | 167.4 |
| RSD | 6.0 | 2.1 | 4.1 | 4.3 | 1.2 |
| 50 ng/mL | |||||
| Intraday Mean | 49.1 | 49.0 | 50.1 | 51.3 | 113.0 |
| RSD | 5.3 | 9.6 | 4.2 | 2.6 | 2.6 |
| Interday Mean | 50.4 | 49.0 | 51.1 | 52.2 | 118.6 |
| RSD | 4.6 | 2.0 | 3.3 | 8.4 | 1.3 |
| 10 ng/mL | |||||
| Intraday Mean | 9.6 | 9.01 | 10.0 | 8.1 | 77.1 |
| RSD | 5.4 | 8.0 | 5.4 | 10.1 | 3.0 |
a
T4 and T3 values are the sum of spiked levels and endogenous levels.
72.4±7.0 ng/mL of T4 and 3.68 ±0.25 ng/g T3 were detected in bovine serum samples, and the values were different from those in Table 4 due to serum samples were from different batches.