Skip to main content
. 2011 May 15;138(10):1977–1989. doi: 10.1242/dev.061341

Fig. 2.

Fig. 2.

Residual Smad4 in the buccal mesenchyme of Osr2-IresCre;Smad4fl/fl mice mediates BMP signaling to induce Msx1 expression. (A-F) Immunofluorescence of β-gal (green in A,B,E,F) and SMAD4 (red in C-F) in control (Osr2-IresCre;Smad4fl/+;R26R)and Osr2-IresCre;Smad4fl/fl;R26R tooth germs at E12.5. Arrowheads and arrows point to the remaining SMAD4 expression in the buccal mesenchyme and to the absence of SMAD4 expression in the lingual mesenchyme, respectively, of Osr2-IresCre;Smad4fl/fl;R26R mice. Broken lines indicate the basement membrane. (G,H) Schematic of SMAD4 expression in the dental epithelium (yellow) and mesenchyme (red) of control (G) and Osr2-IresCre;Smad4fl/fl (H) mice at E12.5. (I,J) In situ hybridization for Msx1 in control (I) and Osr2-IresCre;Smad4fl/fl (J) tooth germs at E13.5. B, buccal; L, lingual; DE, dental epithelium; DM, dental mesenchyme. Scale bars: 50 μm in A-F; 100 μm in I,J.