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. 2011 May 15;138(10):1977–1989. doi: 10.1242/dev.061341

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Fig. 4. — Ectopic bone formation in Osr2-IresCre;Smad4^fl/fl mice. (A-F) Macroscopic views (A,B), H&E staining (C,D) and SEM analysis (E,F) of E11.5 control and Osr2-IresCre;Smad4^fl/fl molar tooth germs after 19 days kidney capsule transplantation. (G-N″) In situ hybridization analysis of odontoblast, osteoblast and ameloblast differentiation markers in E11.5 control (G-J′) and Osr2-IresCre;Smad4^fl/fl (K-N″) molar tooth germs after 19 days kidney capsule transplantation. Boxed areas are shown magnified to the right. Black arrows indicate expression of Dspp, Bsp or Amelx, whereas white arrows indicate lack of detectable expression. (O) qPCR for differentiation markers expressed by odontoblasts (Dspp and Nes) and osteoblasts (Bsp) using dental papilla from newborn (NB) control and Osr2-IresCre;Smad4^fl/fl mice and E11.5 samples after 19 days kidney capsule transplantation. Values are expressed relative to control. *, P<0.05; **, P<0.01; ***, P<0.001. Error bars indicate s.d. AB, alveolar bone; AM, ameloblast; D, dentin; E, enamel; M1, lower first molar; M2, lower second molar; OD, odontoblast. Scale bars: 200 μm in G-N; 50 μm in C,D,G′-N′,K″-N″; 10 μm in E,F.