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. 2011 May 15;138(10):2133–2142. doi: 10.1242/dev.057372

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Fig. 3. — seh1 promotes the maintenance of the oocyte fate. (A,C) Wild-type (A) and seh1^Δ¹⁵ (C) Drosophila ovarioles stained with DAPI and the oocyte markers anti-C(3)G (red) and anti-Orb (green). Note that in a fraction of egg chambers from seh1^Δ¹⁵ ovarioles the oocyte becomes polyploid. (B) A wild-type stage 6 egg chamber that contains 15 polyploid cells. Arrow indicates the oocyte (Oo) DNA that has condensed into a compact karyosome (circled). (D) A seh1^Δ¹⁵ stage 6 egg chamber that contains 16 polyploid cells and no apparent oocyte. (E,F) Germarium from wild-type (E) and seh1^Δ¹⁵ homozygous (F) females stained with anti-Orb (green) and anti-C(3)G (red) antibodies. R1, R2 and R3 denote the regions of the germarium.