Fig. 5.

Seh1 promotes the accumulation of Mio. (A) Western blot examining Mio levels from total protein extracts of wild-type (WT), hypomorphic seh1^EP2417 and null seh1^Δ15 flies. α-Tubulin serves as a loading control. Note that the reduction of Mio protein reflects the severity of the seh1 alleles. (B) A reduction in Mio protein is also observed in S2 tissue culture cells after RNAi against the seh1 transcript. Two independent seh1 RNAi targets were used, both of which reduced Mio protein levels compared with the control, which employed a sequence directed against GFP.