Figure 1. CD11b^low macrophages differ in their protein expression signature from CD11b^high macrophages.

Resolving peritoneal exudates were recovered 66 hrs after zymosan A (1 mg) injection into mice, the cells were immuno-stained for Ly-6G, F4/80, and CD11b, and the Ly-6G⁻/F4/80⁺ macrophages were sorted to CD11b^high and CD11b^low populations. The recovered macrophages were lysed and their protein content was analyzed by SDS-PAGE and Western blotting for the indicated proteins. Results are representative blots (A) and mean ± SE of three independent experiments (B).Fold difference in expression was calculated according to the following formula: Densitometric units (DU) of CD11b^high extract/DU of CD11b^low extracts from three experiments. Significant differences by Student's t test between CD11b^high and CD11b^low extracts (* P value< 0.05, ** P value< 0.005, *** P value< 0.001) are indicated.