Fig. 1.

Schematics of constructs used to generate GnRH-Otx2KO mice. Transgenic mice expressing Cre recombinase specifically in GnRH neurons (A) were bred with Otx2 floxed mice (B). A frt recombination site (white triangle) remains from the neomycin excision downstream of the loxP sites (black triangles). Primers used in PCR genotyping are labeled F1 and R1. PCR primers used to detect recombination are labeled P1 and R1. C, Genotyping by PCR analysis of the genomic DNA produced a band migrating at 560 bp in the mice bearing a FL allele and a band at 360 bp in WT mice. D, PCR analysis with primers P1 and R1 yields a band of 300 bp after recombination. This band was seen only in the hypothalamus (H) and not kidney (K), heart (M), pituitary (P), or cerebellum (C) of the GnRH-Otx2KO mice.