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. 2011 Mar 24;25(5):833–846. doi: 10.1210/me.2010-0271

Fig. 2.

Fig. 2.

The total number and distribution of GnRH neurons in the adult brain of GnRH-Otx2KO mice. A, Histogram of the spatial distribution of GnRH immunostaining neurons from control and GnRH-Otx2KO (cKO) mice. The most significant reduction of the GnRH immunostaining neurons in the cKO mice occurs at the OVLT. The inset is a schematic representation of a mouse brain, denoting the relative location of the OVLT and the median eminence. Each bar spans 120 μm, with error bars representing the sem. B, GnRH immunostaining neurons from representative control (i) and cKO (ii) coronal sections at the OVLT. Note the decrease in GnRH neuronal cell bodies and the axonal projections in the cKO mice. Magnification, ×50. Scale bar, 200 μm. C, The total number of GnRH immunostaining neurons are significantly reduced in the cKO mice. *, P < 0.05; ***, P < 0.0001.