Fig. 5.

GnRH-Otx2KO mice have an increased rate of programmed cell death along the GnRH neuron migratory route. A, Histogram (±sem) showing significantly more cleaved caspase-3 immunostaining in the olfactory placode of GnRH-Otx2KO (cKO) mice than controls at e14.5, with the majority of apoptotic events occurring at the CP. Cell death was similar in the forebrain of both genotypes. *, P < 0.05. B, Photomicrographs of 40-μm sagittal sections of control (i) and GnRH-Otx2cKO (ii) mice immunostained for cleaved caspase-3 at e14.5. Apoptotic cells can be seen along the migratory route from the OP, at the CP, and in the FB region. Magnification, ×200. Scale bar, 50 μm.