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. 2011 Mar 30;5(1):013401. doi: 10.1063/1.3528299

Figure 8.

Figure 8

(a) Schematic diagram of a dual-layer microfluidic device. The fluidic layer is shown in red and the push-down valves are shown in blue. (b) Phase contrast image of single-cell colonies seeded inside the microfluidic device. (c) The single-cell colonies where DAPI indicates staining of dead cell (the arrows). (d) Phase contrast image of a small multicellular colony inside the microfluidic device. (e) DAPI image of a multicellular colony stained with CellTrackert. Additional details regarding the experimental procedures and results can be found in Ref. 139. [Reprinted with permission from D. Ellison et al., Mol. BioSyst. 5, 1004 (2009). © 2009, The Royal Society of Chemistry.]