Figure 2.

Reagent microprinting on cells. (a) The denser DEX phase is used as the patterning phase and printed on cells immersed in the PEG phase. (b) The ATPS microprinting technique allows generating user-defined stable patterns of reagents on cells. (c) Long-term stability of patterns is demonstrated by including liposomal transfection complexes of a dsRed plasmid in the patterning DEX phase and printing them in the shape of a diamond. The resulting gene expression pattern resembles the printed diamond shape. Scale bar 1 mm in (b) and (c). [Panels (b) and (c) adapted with permission from NPG (Ref. ²⁸)].