Figure 1.

Different configurations for studying paracrine and physical interactions between two different cell populations (green and orange). Conventional macroscale approaches without intercellular distance control: seeding in (a) 2D and (c) 3D. Micropatterning approaches with intercellular distance control: (b) 2D and (d) 3D. Microfluidic cell culture of different cell populations: (e) in 3D scaffolds under microenvironmental control and (f) for tissue specimen culture or forming organized cellular structures.