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. Author manuscript; available in PMC: 2011 Apr 26.
Published in final edited form as: J Immunol. 2009 Jun 1;182(11):6659–6669. doi: 10.4049/jimmunol.0804211

FIGURE 1.

FIGURE 1

LAG-3 and PD-1 subsets have distinct phenotype and cytokine secretion patterns. A, Axial LN were harvested 3 days after transfer of Clone 4 CD8 T cells and single cell suspensions were stimulated in vitro with HA peptide and brefeldin A for 4 h. Cells were gated on Thy1.1+ cells to differentiate endogenous from transferred cells. Also shown are LAG-3 knockout Clone 4 CD8 cells as a gating control for LAG-3 as well as WT Clone 4 CD8 stained with LAG-3 and a PD-1 isotype Ab. B, IFN-γ and TNF-α analysis of LAG-3+PD-1int, LAG-3negPD-1int, and PD-1high CD8+Thy1.1+ populations after transfer into C3-HAhigh mice followed by in vitro stimulation. IFN-γ (C), TNF-α (D), ICOS (E), and 4-1BB (F) expression by CD8 subpopulations from C3-HAhigh axial lymph nodes. Shown are representative plots from one of four experiments each, involving three mice per group.