Table 7.1.
Termini of tailed-phage virion DNAs.
Terminus type | Prototype phage | Replication strategy |
---|---|---|
Cohesive ends | ||
5′-single-strand extension | λ P2 | Rolling circle→concatemer |
Circle→circle | ||
3′-single-strand extension | HK97 | Rolling circle→ concatemer* |
Circularly permuted direct terminal repeats† | ||
T4 | Complex→concatemer | |
P22 | Rolling circle→concatemer | |
P1 | Rolling circle→concatemer | |
Host DNA at termini | ||
Mu | Duplicative transposition into host DNA | |
Exact direct terminal repeats | ||
Short (few hundred base pairs) | T7 | Linear→concatemer |
Long (thousands of base pairs) | SPO1 | Complex→concatemer |
T5 | Complex→concatemer | |
Covalent terminal protein | ||
ϕ29 | Protein-primed linear→linear |
Individual virions of these phages have chromosomes that terminate at many different places on the genome sequence, and the length of the terminal repeat varies among individual virions (see text).
Genomic analysis predicts this mode of replication, but it has not been studied experimentally.