Fig. 1.
Effect of green tea extract and catechins on OATP-mediated estrone-3-sulfate uptake. Cells were coincubated with 0.1 μM [3H]estrone-3-sulfate (E3S) and 0.03 μg/ml green tea extract, 100 μM EC, EGC, ECG, EGCG, or the vehicle control (1% DMSO) at 37°C for 20 s [OATP1B1 (B), OATP1B3 (C), and OATP2B1 (D)] or 30 s [OATP1A2 (A)]. After correction for protein, uptake into empty vector (OATP1A2 and OATP2B1) or wild-type control cells (OATP1B1 and OATP1B3) was subtracted to determine OATP-mediated uptake. Values are expressed as a percentage of vehicle control; each value is the mean ± S.E.M. of three independent experiments. Asterisks represent statistically significant differences from the DMSO control (**, p < 0.005; ***, p < 0.001).