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Concentration-dependent binding of corticosteroids to natural melanin. Various concentrations of corticosteroids in solution (0.01–30 μM; n = 3 for each concentration) were incubated as a cocktail with natural melanin (from S. officinalis) dispersed in PBS (pH 7.4), for 6 h at 37°C. At the end of the study, mean bound and free drug concentrations were estimated and plotted.
