TABLE 1.

Amount and calculated concentration of isoflurane in mouse gallbladder

Isoflurane content of mouse gallbladder bile was measured by LC-MS. See Liquid Chromatography-Mass Spectrometry for details.

	Gallbladder Weight	Gallbladder Isoflurane	Calculated Isoflurane Conc.a
	mg	μg	mM
No isoflurane control	4.9	N.D.	N.D.
Emptied gallbladder control	3.3	N.D.	N.D.
Isoflurane-treated mouse 1	26.0	4.72	2.2
Isoflurane-treated mouse 2	12.6	3.25	1.3
Isoflurane-treated mouse 3	17.3	3.52	1.4

N.D., not detected.

^a The calculated isoflurane concentration assumes mouse gallbladder volume of 8 μl/16 g mouse total body weight (Graewin et al., 2004).