. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: J Biomol Screen. 2010 Jul 26;15(8):907–917. doi: 10.1177/1087057110375848

Table 1.

Wee1 degradation assay protocol in 1,536 well plate format

Order	Step	Condition	Comments
1	Cell dispensing	5 μL/well	4,000 cells/well
2	Primary incubation time	4 hours	37°C, 95% R.H., 5% CO₂
3	Compound addition	25 nL/well	Test concentration: 5 μM Final DMSO concentration: 0.5%
4	Secondary incubation time	20 hours	37°C, 95% R.H., 5% CO₂
5	Luciferase detection reagent addition	5 μL/well
6	Tertiary incubation	15 min	Room temperature
7	Luminescence acquisition	60 s/plate