Table 2.
uHTS campaign summary and results
| Step | Screen type | Target | Number of compound tested | Selection criteria | Number of selected compounds | PubChem AIDc | Assay statistics |
|
|---|---|---|---|---|---|---|---|---|
| Z′ | S/B | |||||||
| 1 | Primary screen | Wee1 | 218,117 | %inh > 8.82%a | 2,610 | 1321 | 0.65±0.05 | 17.76±2.68 |
| 2 | Confirmation | Wee1 | 1,090 | %inh > 8.82%b | 39 | 1410 | 0.62±0.02 | 8.42±0.39 |
| 3a | Titration | Wee1 | 1412 | 0.62±0.03 | 8.23±1.01 | |||
| 3b | Selectivity | N-Cyclin B | 38 | %inh>50% in Wee1 and <50% in N-cyclin B | 4 | 1414 | 0.52±0.03 | 6.61±0.25 |
| 3c | Cytotoxicity | N.A. | 1413 | 0.78±0.02 | 38.23±2.26 | |||
a
The primary screen hit-cutoff was calculated at the average percent activation of all test compounds plus three times the standard deviation.
b
The hit-cutoff calculated for the primary run was also applied to the confirmation run.
c
PubChem AIDs are accessible on-line at http://www.ncbi.nlm.nih.gov/sites/entrez?db=pcassay&term=xxxx, where xxxx represents the PubChem AID number listed in the table.
N.A., not applicable