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. Author manuscript; available in PMC: 2011 Apr 26.
Published in final edited form as: Dev Cell. 2005 Aug;9(2):249–259. doi: 10.1016/j.devcel.2005.06.007

Figure 3. Sperm from sAC Null Animals Show an Aberrant Protein Tyrosine Phosphorylation Pattern.

Figure 3

Sperm from +/+ and −/− animals were collected. Both noncapacitated (NC) and capacitated (CAP) sperm were prepared in the presence and absence of 1 mM dbcAMP. Proteins were solubilized, separated by gel electrophoresis, and assayed for the presence of protein tyrosine phosphorylation by immunoblotting with anti-phosphotyrosine antibody.

(A) Protein from +/+ sperm. The band at Mr ~116,000 (arrow) is hexokinase that is constitutively tyrosine-phosphorylated (Kalab et al., 1994).

(B) Protein from −/− sperm. Both noncapacitated and capacitated sperm from −/− animals had an aberrant pattern of protein tyrosine phosphorylation pattern that did not change with the addition of dbcAMP.