Digoxygenin-labeled antisense chordin RNA was hybridized to embryos at stage 9.5 (A); stage 10.25 (B); stage 10.75 (C); stage 11.5 (D); stage 13, note expression in the prechordal plate and notochord (E); stage 26 (F); stage 33, note expression in the chordoneural hinge of the tailbud (G); stage 33, enlarged view of the tailbud region (G’); stage 42, enlarged view of the tail region (H); LiCl-treated embryo at stage 11.5 (I), compare with (D). (A)–(D) and (I) are vegetal views, dorsal side is at the top. (E) is viewed from the dorsal side with anterior at top. (F)–(H) are lateral views.