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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Cold Spring Harb Protoc. 2011 Jan 1;2011:pdb.prot5546. doi: 10.1101/pdb.prot5546
Reagent (for 1200 mL) Quantity
Ethyl 3-aminobenzoate methane-sulfonate salt (0.5 mM) (Sigma-Aldrich) <!> 5 g
Distilled deionized water 1174.8 mL
Tris base <!> (1 M, pH 9.0) 25.2 mL