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. 2011 Apr 26;6(4):e14807. doi: 10.1371/journal.pone.0014807

Figure 2. Physical characterization of the interaction between fascin 2b and filamentous actin.

Figure 2

Negative staining and electron microscopy reveal actin bundling by recombinant fascin 2b fusion protein. Micrographs display filamentous actin in the absence of MBP-fascin 2b (A) and in the presence of MBP-fascin 2b (B); bundled filaments are observed in B. Scale bars are 100 nm. An immunoblot (C, top), from a cosedimentation experiment, shows the progressive depletion of MBP-fascin 2b from supernatants incubated with increasing concentrations of actin; molar concentration (C, middle) of actin for each lane is displayed. Image of a gel (C, bottom), after SDS-PAGE and exposure to SYPRO Ruby protein gel stain, shows the actin found in pellets from a cosedimentation experiment. Plotted are the means of specific equilibrium-binding measurements of MBP-fascin 2b to filamentous actin (D). Each point is a mean ± SEM (number of experiments, N = 4) or an average of two experiments (no error bars).