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. 2011 Apr 26;6(4):e18900. doi: 10.1371/journal.pone.0018900

Figure 8. Expression of IFP fusion proteins in eukaryotic cells.

Figure 8

Proteins extracted from (A) Kluyveromyces lactis, (B) Pichia pastoris and (C) Leishmania tarentolae were separated by SDS-PAGE and analysed by in-gel infrared imaging at 700 nm to detect IFP moieties (upper panels), followed by western transfer and immunological detection at 800 nm (using monoclonal mouse antibody directed against the 6xHis epitope; lower panels). Supernatant (S) and pellet (P) fractions of disrupted cells were used for analysis after ultracentrifugation. Detected or expected protein bands are labelled by dashed frames or asterisks, respectively. IFP fusion proteins present in pellet fractions from P. pastoris did not separate as distinct bands. M, molecular mass marker (kDa).