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. 2011 Apr 26;6(4):e19194. doi: 10.1371/journal.pone.0019194

Figure 7. Reduced NAD+ levels contributes to reduced Sirt1 activity in the liver, heart, kidney and lung with aging.

Figure 7

(A) Reduced Sirt1 activity was observed in the rat liver, heart, kidney and lung after 12 months of age using a flourometry. All values are means ± S.E from tissue obtained from eight different rats for each age group. Significance *p<0.01 compared to 3 month old rats. (B) Western blotting for Sirt1 in (i) liver, (ii) heart, (iii) kidney, and (iv) lung with aging using anti-Sirt1 antibody. The blots shown are representative tracings of an experiment done eight times. Graphs are mean ± S.E brains from tissue obtained from eight different rats for each age group. Each bar of the quantification graph represents the corresponding band for each age group. Significance *p<0.01 compared to 3 month old rats. (C) Immunodetection for Sirt1 in the liver, heart, kidney and lung from 3 month, 12 month and 24 month old rats. Sirt1 (red) and DAPI (blue). Higher immunoreactivity for Sirt1 was observed in 12 and 24 month old rats compared to 3-month old rats.