Figure 8. Reduced Sirt1 activity induces p53 acetylation in the liver, heart, kidney and lung with aging.

Acetylated p53 and total p53 levels were determined by Western blotting in (A) liver, (B) heart, (C) kidney, and (D) lung with aging using anti-acetylated p53 and anti-total p53 antibodies. The blots shown are representative tracings of an experiment done eight times. Graphs are mean ± S.E brains from tissue obtained from eight different rats for each age group. Each bar of the quantification graph represents the corresponding band for each age group. Significance ^*p<0.01 compared to 3 month old rats.