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. 2011 Apr 26;6(4):e19194. doi: 10.1371/journal.pone.0019194

Table 1. List of primary antibodies used.

Antibody Type Antigen Dilution Source
(E)-4-Hydroxynonenal Polyclonal Free HNE (E)-4-hydroxynonenal 1∶1000 Alexis Biochemicals
Phospho-H2AX-Serine139 Polyclonal Phosphorylated H2AX at Serine 139 1∶1000 Alexis Biochemicals
Poly(ADP-ribose) (10H) Monoclonal Poly(ADP-ribose) 1∶400 Alexis Biochemicals
Sirt1 Polyclonal Sirt1 1∶2000 Alexis Biochemicals
Acetyl K386 Polyclonal Acetylated p53 at lysine 386 1∶1000 Abcam
p53 Oncogene Monoclonal Total p53 oncogene 1∶1000 Millipore