Table 1. List of primary antibodies used.
Antibody | Type | Antigen | Dilution | Source |
(E)-4-Hydroxynonenal | Polyclonal | Free HNE (E)-4-hydroxynonenal | 1∶1000 | Alexis Biochemicals |
Phospho-H2AX-Serine139 | Polyclonal | Phosphorylated H2AX at Serine 139 | 1∶1000 | Alexis Biochemicals |
Poly(ADP-ribose) (10H) | Monoclonal | Poly(ADP-ribose) | 1∶400 | Alexis Biochemicals |
Sirt1 | Polyclonal | Sirt1 | 1∶2000 | Alexis Biochemicals |
Acetyl K386 | Polyclonal | Acetylated p53 at lysine 386 | 1∶1000 | Abcam |
p53 Oncogene | Monoclonal | Total p53 oncogene | 1∶1000 | Millipore |