Table 2. Assay of TIC frequencies for Lrp5⁺ and Lrp⁻ cell subpopulations from Wnt-induced tumors.

Cell fraction	% cells in fraction	# of cells transferred	Take rate	Frequency of TICs (95% CI) (pGOF)	TICs per 106 total cells (Lin−)	Fold enrichment
Lrp5+	36.7	2000	4/4	1/386*	951	2.4
		1000	2/2	(1/178–1/838) (0.87)
		500	4/6
		100	1/4
Lrp5−	38.4	3000	3/4	1/4426*	87	0.2
		2000	1/4	(1/1862–1/10530) (0.106)
		1500	1/2
		1000	0/4
		500	0/4

Lrp5⁺ and Lrp5⁻ (Lin−) cell fractions were purified by flow cytometry, assayed by isograft to mammary fat pads at limiting dilutions, and results were reported as for Table 1. Pairwise comparison of the Lrp+ and Lrp− cells shows that the TIC frequency is significantly different (* p = 2.34×10⁻⁵).