Fig. 1.
In vitro differentiation of isolated CD14+ monocytes to immature dendritic cells (DC). CD14+ cells were treated with GM-CSF/IL-4 for 6 days, during which CD14, HLA-DR, and CD86 expression was analyzed by flow cytometry. (A) Forward and side scatter of differentiating DC was determined on days 0, 2, 4 and 6. This same population of CD14+ monocytes lose CD14 expression over time (B), while showing increased expression of HLA-DR and CD86 (C) during conversion to DC.


