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. Author manuscript; available in PMC: 2012 Jun 1.

Published in final edited form as: Am J Reprod Immunol. 2011 Jan 18;65(6):597–609. doi: 10.1111/j.1600-0897.2010.00968.x

Fig. 4 — Differential cytokine profile of CD8+ T cells following co-culture with Ag-loaded DC. DC were loaded with different Ag forms [NP=empty nanoparticle; L= lysate; NPL= NP-lysate] and co-cultured with autologous CD8 T-cells for 72 hrs, after which cell-free conditioned media (CM) was analyzed for cytokine production. (A) CD8 T cells co-cultured with DC loaded with NPL produce a pro-inflammatory/Th1 cytokine profile characterized by specific induction of IL-1β, IL-6, IFNγ, TNFα, G-CSF and RANTES. (B) CD8 T cells co-cultured with DC loaded with soluble lysate produce a weakly Th2 or potentially tolergenic profile.

Fig. 4 — Differential cytokine profile of CD8+ T cells following co-culture with Ag-loaded DC. DC were loaded with different Ag forms [NP=empty nanoparticle; L= lysate; NPL= NP-lysate] and co-cultured with autologous CD8 T-cells for 72 hrs, after which cell-free conditioned media (CM) was analyzed for cytokine production. (A) CD8 T cells co-cultured with DC loaded with NPL produce a pro-inflammatory/Th1 cytokine profile characterized by specific induction of IL-1β, IL-6, IFNγ, TNFα, G-CSF and RANTES. (B) CD8 T cells co-cultured with DC loaded with soluble lysate produce a weakly Th2 or potentially tolergenic profile.